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Invitrogen
LAIR1 Monoclonal Antibody (NKTA255), Alexa Fluor™ 488
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FIGURE: 1 / 3
LAIR1 Antibody (MA5-44135) in Flow
Separation of human CD305 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 Monoclonal antibody (Product # MA5-44135) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.
Product Details
MA5-44135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
NKTA255
Immunogen
Activated NK cells and CD3- thymocytes
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.03 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.
Target Information
CD305, also known as LAIR1, is a type I transmembrane glycoprotein with an extracellular Ig-like domain and two intracellular ITIMs. It is expressed on a wide range of immune cells, including T, NK, and B cells, monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, CD305 is also present on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms exist due to alternative splicing, with expression varying between cell types. CD305 functions as an inhibitory receptor, playing a key role in regulating immune responses. It blocks cytokine-mediated signaling, cell proliferation, and apoptosis. In NK, B, and T cells, CD305 modulates cytolytic functions and cytokine production, down-regulating IL2 and IFNG in CD4+ T cells while inducing transforming growth factor beta. In B cells, it reduces IgG and IgE production and decreases IL8, IL10, and TNF secretion. Activation of CD305 through tyrosine phosphorylation recruits phosphatases PTPN6 and PTPN11, although it may also function independently of SH2-containing phosphatases. It inhibits differentiation of peripheral blood precursors into dendritic cells and has been shown to induce apoptosis in myeloid leukemia cell lines. Collagens are identified as ligands for CD305, underscoring its role in immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD305; immunoglobulin heavy chain variable region; LAIR-1; LAIR1; leukocyte-associated Ig-like receptor 1; Leukocyte-associated immunoglobulin-like receptor 1; membrane glycoprotein; unnamed protein product
Gene Aliases: CD305; LAIR-1; LAIR1
UniProt ID: (Human) Q6GTX8
Entrez Gene ID: (Human) 3903
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