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Perforin Monoclonal Antibody (dG9), PE
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA574386
Product Specifications
Species Reactivity
Bovine,
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
dG9
Immunogen
purified granules from human YT lymphoma cell line.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytolysin; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; perforin 1 (pore forming protein); Perforin1; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; PRF1 (pore forming protein 1); RP11-710A11.3; unnamed protein product
Gene Aliases: HPLH2; P1; PFP
UniProt ID: (Human) P14222
Entrez Gene ID: (Human) 5551, (Bovine) 369025
immunological synapse formation
leukocyte mediated cytotoxicity
T cell mediated cytotoxicity
defense response to tumor cell
immune system process
immune response to tumor cell
apoptotic process
defense response
cellular defense response
protein secretion
protein import
protein homooligomerization
defense response to virus
positive regulation of killing of cells of other organism
protein transmembrane transport
granzyme-mediated programmed cell death signaling pathway
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