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Invitrogen
MARCH9 Polyclonal Antibody
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MARCH9 Antibody (PA5-88012) in WB
Western blot analysis of MARCH9 in 293T cells. Samples were incubated with MARCH9 Polyclonal antibody (Product # PA5-88012) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-88012
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 277-346 of human MARCH9 (NP_6124052)
偶联物
形式
Liquid
浓度
2.04 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: GTAGKSGPRN SRTGPTSGAT SRPPAAQRMR TLLPQRCGYT ILHLLGQLRP PDARSSSHSG REVVMRVTTV; Positive Samples: U-251MG, A-549, 293T, Mouse intestine, Mouse kidney, Mouse eye, Rat kidney, Rat eye; Cellular Location: Golgi apparatus membrane, Lysosome membrane, Multi-pass membrane protein
靶标信息
MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 9-Mar; E3 ubiquitin-protein ligase MARCHF9; MARCH-IX; membrane associated ring finger 9; membrane-associated ring finger (C3HC4) 9; Membrane-associated RING finger protein 9; Membrane-associated RING-CH protein IX; RING finger protein 179; RING-type E3 ubiquitin transferase MARCHF9
基因别名: MARCH-IX; MARCH9; MARCHF9; RNF179
UniProt ID: (Human) Q86YJ5
Entrez Gene ID: (Human) 92979
