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Invitrogen
MED14 Polyclonal Antibody
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FIGURE: 1 / 1
MED14 Antibody (PA5-79655) in WB
Western blot analysis of MED14 in Lane 1: rat liver tissue lysate, Lane 2: mouse liver tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with MED14 polyclonal antibody (Product # PA5-79655) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79655
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MED14 recombinant protein (Position: D181-D375).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, mouse liver tissue.
Target Information
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activator-recruited cofactor 150 kDa component; ARC150; cofactor required for Sp1 transcriptional activation subunit 2 (150 kDa); cofactor required for Sp1 transcriptional activation, subunit 2; cofactor required for Sp1 transcriptional activation, subunit 2 (150kD); CRSP complex subunit 2; EXLM1; hRGR1; human homolog of yeast RGR1; MGC104513; RGR1 homolog; thyroid hormone receptor-associated protein complex 170 kDa component; thyroid hormone receptor-associated protein complex component TRAP170; transcriptional co-activator CRSP150; unnamed protein product; vitamin D receptor-interacting protein complex component DRIP150; vitamin D3 receptor-interacting protein complex 150 kDa component
Gene Aliases: 9930001L01Rik; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; Gm641; ORF1; RGD1560170; RGR1; TRAP170
UniProt ID: (Human) O60244, (Mouse) A2ABV5
Entrez Gene ID: (Human) 9282, (Rat) 317343, (Mouse) 26896
regulation of transcription from RNA polymerase II promoter
biological_process
stem cell population maintenance
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription initiation from RNA polymerase II promoter
positive regulation of transcription elongation from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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