Invitrogen

MED20 Polyclonal Antibody

Name: MED20 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-90677

查看其他6个TRFP抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

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MED20 Antibody (PA5-90677) in WB

Western blot analysis of MED20 in extracts of various cell lines. Samples were incubated with MED20 Polyclonal antibody (Product # PA5-90677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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Immunogen image

Immunogen Image MED20 Polyclonal Antibody

产品信息

PA5-90677

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_0042662)

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.32 mg/mL

132 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2806170

产品详细信息

Immunogen sequence: MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG FFQSAKASKI ETRGTRYQYC DFLVKVGTVT MGPSARGISV EVEYGPCVVA SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH DAVYGPADTM VQYMELFNKI RKQQQVPVAG IR; Positive Samples: DU145, Mouse brain; Cellular Location: Nucleus

靶标信息

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hTRFP; Mediator complex subunit 20; Mediator of RNA polymerase II transcription subunit 20; predicted protein of HQ0213; Trf (TATA binding protein-related factor)-proximal homolog; Trf (TATA binding protein-related factor)-proximal protein homolog; TRF-proximal protein homolog; TRFP; RNA polymerase II holoenzyme component; ubiquitin specific protease 49; unnamed protein product

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基因别名: 1110011O05Rik; 2410115I17Rik; AU018348; MED20; PRO0213; SRB2; TRFP; Usp49

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UniProt ID: (Human) Q9H944, (Mouse) Q9R0X0, (Rat) Q5XIE9

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Entrez Gene ID: (Human) 9477, (Mouse) 56771, (Rat) 316209

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功能

transcription cofactor activity transcription coactivator activity DNA-directed RNA polymerase activity protein binding RNA polymerase II transcription cofactor activity ubiquitin protein ligase activity ubiquitin-protein ligase

参与通路

transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of transcription elongation from RNA polymerase II promoter skeletal muscle cell differentiation RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription initiation from RNA polymerase II promoter regulation of transcription, DNA-templated protein ubiquitination