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Invitrogen
N-cadherin Monoclonal Antibody (3B9)
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N-cadherin Antibody (33-3900) in ICC/IF
Immunofluorescent analysis of N-Cadherin was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with N-Cadherin Mouse Monoclonal Antibody (Product # 33-3900) at 1 µg - 2 µg in 1% BSA and incubated for 3 hours ... View More
产品信息
33-3900
应用
建议稀释比
已发表文章
产品规格
种属反应
Chicken,
Human,
Mouse,
Pig,
Rat
已发表种属
Avian,
C. elegans,
Dog,
Hamster,
Horse,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Tag,
Zebrafish
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
3B9
抗原
Recombinant domain corresponding to the intracellular domain of chicken N-Cadherin
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific for N-cadherin and does not cross-react with other cadherin family members including P- and E-cadherins.
For IHC with this antibody use formalin-fixed, paraffin-embedded tissue and heat-induced epitope retrieval.
靶标信息
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin; neural calcium-dependent cell adhesion molecule; unnamed protein product
基因别名: cadherin-2; CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD
UniProt ID: (Human) P19022, (Chicken) Q90630, (Mouse) P15116, (Rat) Q9Z1Y3
Entrez Gene ID: (Pig) 100515322, (Human) 1000, (Chicken) 414745, (Mouse) 12558, (Rat) 83501
calcium ion binding
protein binding
beta-catenin binding
protein kinase binding
protein phosphatase binding
protein complex binding
alpha-catenin binding
gamma-catenin binding
nitric-oxide synthase binding
identical protein binding
cadherin binding
extracellular matrix binding
enzyme binding
metal ion binding
cadherin
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
glial cell differentiation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
cerebral cortex development
regulation of myelination
adherens junction organization
regulation of Rho protein signal transduction
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
striated muscle cell differentiation
positive regulation of muscle cell differentiation
protein heterooligomerization
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
cartilage condensation
neural tube formation
synapse assembly
brain development
negative regulation of cell migration
neural crest cell delamination
regulation of synaptic transmission, glutamatergic
limb development
trigeminal ganglion formation
trigeminal ganglion structural organization
protein localization to plasma membrane
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
cell aggregation
regulation of signal transduction
single organismal cell-cell adhesion
regulation of protein localization
