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Invitrogen
N-cadherin Monoclonal Antibody (NCD-2)
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产品信息
13-2100
应用
建议稀释比
已发表文章
产品规格
种属反应
Chicken,
Xenopus
已发表种属
Avian,
C. elegans,
Chicken,
Hamster,
Human,
Mouse,
Xenopus
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
NCD-2
抗原
Neuronal tissue of chick embryos.
偶联物
形式
Lyophilized
浓度
2.0 mg/mL
纯化类型
purified
保存液
PBS, pH 7.4, with 1% BSA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of Wistar rat after immunization with nervous tissue of chick embryos. The monoclonal antibody was harvested from ascitic fluid of SCID mouse.
Specificity: This antibody specifically reacts with chicken N-cadherin. This antibody inhibits N-cadherin dependent cell-cell contact.
Cross reactivity: This antibody reacts with Xenopus N-cadherin. This antibody does not react with human or mouse N-cadherin.
Storage: 4°C This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Ca2+ should be contained in the buffers to stabilize cadherin antigen.
靶标信息
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin-2; Cadherin-2A; Cadherin-N; Cadherin2; CDw325 antigen; N-cadherin; N-cadherin A; N-cadherin-2; Neural cadherin; Neural cadherin A; Neural cadherin-2; neural calcium-dependent cell adhesion molecule; unnamed protein product
基因别名: cadherin-2; CDH2; cdh2-a; cdh2-b; cdh2a; cdhn-a; N-Cad; XELAEV_18031953mg
UniProt ID: (Chicken) Q90630, (Xenopus) P33147
Entrez Gene ID: (Chicken) 414745, (Xenopus) 378518
cartilage condensation
neural tube formation
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
synapse assembly
brain development
glial cell differentiation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
negative regulation of cell migration
neural crest cell delamination
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
striated muscle cell differentiation
regulation of synaptic transmission, glutamatergic
radial glial cell differentiation
limb development
neuroepithelial cell differentiation
trigeminal ganglion formation
trigeminal ganglion structural organization
regulation of oligodendrocyte progenitor proliferation
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
cell aggregation
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
