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Invitrogen
NECAP1 Polyclonal Antibody
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NECAP1 Antibody (PA5-100636) in ICC/IF
Immunocytochemistry analysis of NECAP1 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NECAP1 polyclonal antibody (Product # PA5-100636) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100636
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NECAP1(Accession Q8NC96), corresponding to amino acid residues E8-I58.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total NECAP1.
靶标信息
Involved in endocytosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: adaptin ear-binding clathrin-associated protein; Adaptin ear-binding coat-associated protein 1; adaptin-ear-binding coat-associated protein 1; brain enriched NECAP2 similar protein; clathrin-coated vesicle coat protein; NECAP endocytosis-associated protein 1; NECAP-1; snoRNA MBI-129
基因别名: 1200016B17Rik; AI747569; EIEE21; mFLJ00061; NECAP1; RGD1306053
UniProt ID: (Human) Q8NC96, (Rat) P69682, (Mouse) Q9CR95
Entrez Gene ID: (Human) 25977, (Rat) 312694, (Mouse) 67602
