Invitrogen

NGEF Polyclonal Antibody

Name: NGEF Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-49485
Availability: InStock

查看其他6个NGEF抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

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NGEF Antibody (PA5-49485) in WB

Western blot analysis of NGEF in various lysates. Samples were incubated with NGEF polyclonal antibody (Product # PA5-49485) using a dilution of 1:1,000-1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: A431 whole cell lysates; Lane 2: rat brain lysates. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image NGEF Polyclonal Antibody

产品信息

PA5-49485

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 187-220 amino acids from the N-terminal region of human NGEF.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

100 µg

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2634939

靶标信息

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.

仅用于科研。不用于诊断过程。未经明确授权不得转售。