Invitrogen

NUMB Polyclonal Antibody

Name: NUMB Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-78257

查看其他25个NUMB抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (6)

NUMB Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

NUMB Antibody (PA5-78257) in ICC/IF

Immunofluorescence analysis of NUMB was performed using 70% confluent log phase U87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NUMB Polyclonal Antibody (Product # PA5-78257) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More

Product Image Gallery

Immunogen image

Immunogen Image NUMB Polyclonal Antibody

产品信息

PA5-78257

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the N-terminus region of human NUMB. The exact sequence is proprietary.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2736268

产品详细信息

Positive Control: A431, mouse lung, rat lung

Predicted Reactivity: Zebrafish (88%), Chicken (93%), Bovine (93%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。