Invitrogen

Nogo-B Recombinant Rabbit Monoclonal Antibody (JM02-34)

Name: Nogo-B Recombinant Rabbit Monoclonal Antibody (JM02-34)
Brand: Invitrogen
SKU: MA5-32763

高级验证

查看其他3个Nogo-B抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (11)
高级验证 (1)

Nogo-B Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

Nogo-B Antibody (MA5-32763) in ICC/IF

Immunocytochemical analysis of Nogo-B in SH-SY5Y cells using a Nogo-B Monoclonal antibody (Product # MA5-32763) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Nogo-B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nogo-B Antibody in Flow Cytometry (Flow)

Product Image Gallery

Immunogen image

Immunogen Image Nogo-B Recombinant Rabbit Monoclonal Antibody (JM02-34)

产品信息

MA5-32763

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:1,000

免疫沉淀 (IP)

1:10-1:50

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM02-34

抗原

Synthetic peptide within Human Nogo aa 111-160

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2810040

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Foocen; Human NogoA; My043 protein; neurite growth inhibitor 220; Neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; Nogo protein; NSP; reticulon 5; Reticulon-4; Reticulon-5; RTN-x

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基因别名: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; KIAA0886; mKIAA0886; mKIAA4153; My043; Nbla00271; Nbla10545; NgA; NI220/250; NOGO; NOGO-A; Nogo-B; Nogo-C; NOGOC; NSP; NSP-CL; RTN-X; RTN4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; SP1507

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UniProt ID: (Human) Q9NQC3, (Mouse) Q99P72

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Entrez Gene ID: (Human) 57142, (Mouse) 68585

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功能

protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion protein complex binding

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