Invitrogen

OR51M1 Polyclonal Antibody

查看其他2个OR51M1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

OR51M1 Antibody in Immunocytochemistry (ICC/IF)

OR51M1 Antibody in Immunocytochemistry (ICC/IF)

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OR51M1 Antibody (PA5-100966) in ICC/IF

Immunocytochemistry analysis of OR51M1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with OR51M1 polyclonal antibody (Product # PA5-100966) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

OR51M1 Antibody in Immunocytochemistry (ICC/IF)

OR51M1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OR51M1 Antibody in Western Blot (WB)

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OR51M1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of OR51M1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with OR51M1 polyclonal antibody (Product # PA5-100966) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

OR51M1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of OR51M1 in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with OR51M1 polyclonal antibody (Product # PA5-100966) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

OR51M1 Antibody in Western Blot (WB) — Western blot analysis of OR51M1 in SiHa cells (left lane: treated with the antigen-specific peptide). Samples were incubated with OR51M1 polyclonal antibody (Product # PA5-100966).

Immunogen image

OR51M1 Polyclonal Antibody

Immunogen Image OR51M1 Polyclonal Antibody

产品信息

PA5-100966

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human OR51M1(Accession Q9H341), corresponding to amino acid residues L257-K307.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total OR51M1.

靶标信息

Odorant receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Odorant receptor HOR5'beta7; Olfactory receptor 51M1; Olfactory receptor OR11-40; olfactory receptor, family 51, subfamily M, member 1

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基因别名: HOR5'Beta7; OR11-40; OR51M1

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UniProt ID: (Human) Q9H341

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Entrez Gene ID: (Human) 390059

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G-protein coupled receptor activity olfactory receptor activity transmembrane signal receptor

signal transduction G-protein coupled receptor signaling pathway sensory perception of smell detection of chemical stimulus involved in sensory perception of smell