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Invitrogen
Occludin Polyclonal Antibody
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Occludin Antibody (71-1500) in ICC/IF
Immunofluorescence analysis of Occludin was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Occludin Rabbit Polyclonal Antibody (Product # 71-1500) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More
产品信息
71-1500
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat,
Dog,
Human
已发表种属
Avian,
Baboon,
Bacteria,
Bovine,
C. elegans,
Chicken,
Dog,
Goat,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Sheep,
Zebrafish
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A fusion protein containing the C-terminal 150 amino acid region of human occludin.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein
基因别名: BLCPMG; OCLN; PPP1R115
UniProt ID: (Dog) Q28269, (Human) Q16625, (Rat) Q6P6T5
Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Rat) 83497
positive regulation of lamellipodium assembly
positive regulation of microtubule polymerization
bicellular tight junction assembly
positive regulation of wound healing
protein localization to cell leading edge
protein complex assembly
methylation
cell-cell junction organization
S-adenosylhomocysteine metabolic process
S-adenosylmethionine metabolic process
