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Orexin Polyclonal Antibody
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FIGURE: 1 / 1
Orexin Antibody (PA5-121242) in WB
Western blot analysis of Orexin in extracts of various cell lines. Samples were incubated with Orexin Polyclonal antibody (Product # PA5-121242) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121242
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.21 mg/mL
Amount
21 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: U-251MG, Rat thymus. The target is usually found in the following locations: Cell junction, Cytoplasmic vesicle, Rough endoplasmic reticulum, synapse.
Immunogen sequence: QPLPDCCRQK TCSCRLYELL HGAGNHAAGI LTL
Target Information
Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hypocretin (orexin) neuropeptide; hypocretin neuropeptide; orexin; orexin A; orexin B; precursor of hypocretin neuropeptides; prepro-orexin; preprohypocretin
Gene Aliases: NRCLP1; orexin-A; OX; PPOX
UniProt ID: (Human) O43612, (Rat) O55232, (Mouse) O55241
Entrez Gene ID: (Human) 3060, (Rat) 25723, (Mouse) 15171
temperature homeostasis
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
neuropeptide signaling pathway
chemical synaptic transmission
feeding behavior
negative regulation of DNA replication
sleep
response to starvation
eating behavior
drinking behavior
negative regulation of potassium ion transport
regulation of neurotransmitter secretion
behavioral response to ethanol
positive regulation of calcium ion transport
negative regulation of transmission of nerve impulse
positive regulation of transmission of nerve impulse
excitatory postsynaptic potential
response to alcohol
positive regulation of cold-induced thermogenesis
response to peptide
behavioral fear response
release of sequestered calcium ion into cytosol
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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