Invitrogen

P-Glycoprotein Monoclonal Antibody (C219)

Name: P-Glycoprotein Monoclonal Antibody (C219)
Brand: Invitrogen
SKU: MA1-26528

高级验证

14个已发表图片

46 篇参考文献

查看其他40个P-Glycoprotein (ABCB1)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

P-Glycoprotein Antibody in Western Blot (WB)

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P-Glycoprotein Antibody (MA1-26528) in WB

Western blot was performed using Anti-P-Glycoprotein Monoclonal Antibody (C219) (Product # MA1-26528) and due to glycosylation, 160-230 kDa band corresponding to ABCB1 was observed across cell lines tested except MCF-7 which is reported to be negative. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Caco-2 (Lane 2), MDCK (Lane 3) and MCF-7 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell Sur... View More

P-Glycoprotein Antibody in Immunocytochemistry (ICC/IF)

P-Glycoprotein Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image P-Glycoprotein Monoclonal Antibody (C219)

产品信息

MA1-26528

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50-1:500

查看28篇已发表文章 28篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

≥1:25

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

查看7篇已发表文章 7篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

亲和纯化 (AP)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Bovine, Dog, Fish, Human, Mouse, Rat, Zebrafish

已发表种属

C. elegans, Dog, Fish, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

C219

抗原

SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

纯化类型

purified

保存液

PBS with 1% BSA

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_795165

产品详细信息

Recommended positive controls: Cell lines or frozen tissue, such as liver or colon.

MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

This clone recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein (~170kDa) and MDR3 P-glycoprotein (~140kDa). In Western blot, boiling samples may reduce visibility of the protein.

For flow cytometry, cells should be fixed in 3.7% formaldehyde for 10 min at room temperature to permeabilize the cell membrane. Incubate cells with 5-10 µg antibody for 30-60 min at 4°C.

靶标信息

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ABC superfamily; P-glycoprotein; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; CD243; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; MDR1A; multi-drug resistance 3; multidrug resistance p-glycoprotein; Multidrug resistance protein 1; Multidrug resistance protein 1A; Multidrug resistance protein 1B; Multidrug resistance protein 3; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein; P-glycoprotein 1; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Phospholipid transporter ABCB1

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基因别名: ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; CD243; CLCS; Evi32; GP170; mdr; mdr-3; MDR1; Mdr1a; Mdr1b; Mdr3; P-GP; Pgp; Pgy-1; Pgy-3; PGY1; Pgy1-1; Pgy3

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UniProt ID: (Human) P08183, (Mouse) P21447, (Mouse) P06795