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Invitrogen
PARP1 (cleaved Asp214, Asp215) Polyclonal Antibody
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产品信息
44-698G
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. The antibody has been used in western blotting and previous lots have been used in immunostaining applications. Positive controls used in western blotting were Jurkat or HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).
靶标信息
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1; unnamed protein product
基因别名: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1
UniProt ID: (Bovine) P18493, (Human) P09874, (Rat) P27008
Entrez Gene ID: (Bovine) 286764, (Human) 142, (Rat) 25591, (Mouse) 11545
DNA binding
zinc ion binding
NAD binding
protein ADP-ribosylase activity
DNA ligase (ATP) activity
NAD+ ADP-ribosyltransferase activity
protein binding
transcription factor binding
enzyme binding
protein kinase binding
identical protein binding
histone deacetylase binding
poly(A) RNA binding
protein N-terminus binding
R-SMAD binding
transferase activity, transferring pentosyl groups
chromatin binding
transferase activity
transferase activity, transferring glycosyl groups
metal ion binding
DNA metabolism protein
double-strand break repair
protein ADP-ribosylation
cellular response to DNA damage stimulus
positive regulation of cardiac muscle hypertrophy
peptidyl-serine ADP-ribosylation
peptidyl-glutamic acid poly-ADP-ribosylation
DNA ADP-ribosylation
protein poly-ADP-ribosylation
protein auto-ADP-ribosylation
positive regulation of double-strand break repair via homologous recombination
ATP generation from poly-ADP-D-ribose
negative regulation of transcription from RNA polymerase II promoter
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
double-strand break repair via homologous recombination
lagging strand elongation
DNA repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
transcription from RNA polymerase II promoter
mitochondrion organization
transforming growth factor beta receptor signaling pathway
protein autoprocessing
protein sumoylation
signal transduction involved in regulation of gene expression
macrophage differentiation
mitochondrial DNA metabolic process
cellular response to insulin stimulus
nucleotide-excision repair, DNA incision
cellular response to oxidative stress
protein modification process
DNA damage response, detection of DNA damage
mitochondrial DNA repair
positive regulation of transcription from RNA polymerase II promoter
DNA ligation involved in DNA repair
positive regulation of SMAD protein import into nucleus
global genome nucleotide-excision repair
regulation of cellular protein localization
negative regulation of telomere maintenance via telomere lengthening
positive regulation of transcription regulatory region DNA binding
telomere maintenance
DNA metabolic process
base-excision repair
transcription, DNA-templated
regulation of growth rate
cellular response to superoxide
regulation of transcription, DNA-templated
behavioral response to cocaine
regulation of single strand break repair
