Invitrogen

PDI Monoclonal Antibody (RL90), Alexa Fluor™ 488

Name: PDI Monoclonal Antibody (RL90), Alexa Fluor\u2122 488
Brand: Invitrogen
SKU: MA3-019-A488
Availability: InStock

8个已发表图片

3 篇参考文献

查看其他31个PDI抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)
已发表图片 (8)

PDI Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

PDI Antibody (MA3-019-A488) in ICC/IF

Immunofluorescent analysis of PDI (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a PDI Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA3-019-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images w... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

MA3-019-A488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1-10 µg/mL

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Hamster, Human, Mouse, Pig, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

RL90

抗原

Purified rat PDI protein

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

50 µg

纯化类型

Protein A

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2662487

产品详细信息

MA3019A488 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.

The MA3019A488 antigen is purified rat PDI.

靶标信息

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CaO19.12595; Cellular thyroid hormone-binding protein; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; p55; p58; PDI; PDI1P; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); Protein disulfide-isomerase

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基因别名: ERBA2L; ERp59; I79_002399; P4HB; p58; PDI; PDIA1; PDIR; PO4DB; Thbp