Invitrogen

PPAR gamma-2 Polyclonal Antibody

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19 篇参考文献
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说明书
实验方案
问题与答案
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PPAR gamma-2 Polyclonal Antibody

产品信息

PA1-824

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500
查看10篇已发表文章 10篇已发表文章

免疫组化 (IHC)

-
查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:500
查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

-
查看1篇已发表文章 1篇已发表文章

凝胶阻滞分析 (GS)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2166200

产品详细信息

PA1-824 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human and mouse tissues. This antibody does not detect PPAR alpha or PPAR delta.

PA1-824 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate. PA1-824 inhibits PPAR gamma 2 DNA binding.

PA1-824 immunogen is a synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2. This sequence is not conserved in PPAR gamma-1.

PA1-824 can be used with blocking peptide PEP-027.

靶标信息

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR-gamma

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基因别名: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2

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UniProt ID: (Human) P37231, (Mouse) P37238

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Entrez Gene ID: (Human) 5468, (Mouse) 19016

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功能
core promoter sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding retinoid X receptor binding arachidonic acid binding alpha-actinin binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding metal ion binding WW domain binding C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator
参与通路
negative regulation of transcription from RNA polymerase II promoter placenta development negative regulation of acute inflammatory response transcription initiation from RNA polymerase II promoter lipid metabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction G-protein coupled receptor signaling pathway heart development response to nutrient regulation of blood pressure response to cold response to mechanical stimulus macrophage derived foam cell differentiation negative regulation of macrophage derived foam cell differentiation negative regulation of receptor biosynthetic process negative regulation of cholesterol storage negative regulation of sequestering of triglyceride long-chain fatty acid transport fatty acid oxidation monocyte differentiation negative regulation of cell growth epithelial cell differentiation response to caffeine organ regeneration response to retinoic acid cellular response to insulin stimulus negative regulation of collagen biosynthetic process response to vitamin A response to lipid peroxisome proliferator activated receptor signaling pathway response to immobilization stress response to diuretic glucose homeostasis response to starvation regulation of circadian rhythm lipoprotein transport steroid hormone mediated signaling pathway response to estrogen innate immune response cell fate commitment positive regulation of fat cell differentiation low-density lipoprotein particle receptor biosynthetic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation cell maturation rhythmic process negative regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation white fat cell differentiation positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of telomerase activity lipid homeostasis response to low-density lipoprotein particle positive regulation of phagocytosis, engulfment negative regulation of interferon-gamma-mediated signaling pathway regulation of cholesterol transporter activity regulation of transcription involved in cell fate commitment cellular response to retinoic acid cellular response to vitamin E cellular response to prostaglandin E stimulus cellular response to hyperoxia response to metformin negative regulation of pancreatic stellate cell proliferation negative regulation of cytokine production response to dietary excess diet induced thermogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response negative regulation of cell proliferation response to light stimulus regulation of gene expression regulation of lipid metabolic process intracellular receptor signaling pathway response to food positive regulation of apoptotic process fat cell differentiation regulation of fat cell differentiation brown fat cell differentiation cellular response to lithium ion cellular response to organic cyclic compound negative regulation of peptide hormone secretion negative regulation of cellular response to insulin stimulus
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