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Invitrogen
PSA-NCAM Monoclonal Antibody (12E3), eBioscience™
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PSA-NCAM Antibody (14-9118-82) in ICC/IF
Immunofluorescence analysis of PSA-NCAM was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSA-NCAM Monoclonal Antibody (12E3), eBioscience (Product # 14-9118-82) at 20 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... View More
产品信息
14-9118-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
12E3
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody 12E3 specifically recognizes polysialylated neural cell adhesion molecule (PSA-NCAM) in human, mouse and rat. Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.
Applications Reported: This 12E3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This 12E3 antibody has been tested by immunocytochemistry of formaldehyde-fixed PC-12 (rat) cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: antigen recognized by monoclonal antibody 5.1H11; CD56; N-CAM-1; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; Polysialylated NCAM; PSA NCAM
基因别名: CD56; E-NCAM; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC
UniProt ID: (Human) P13591, (Mouse) P13595, (Rat) P13596
Entrez Gene ID: (Human) 4684, (Mouse) 17967, (Rat) 24586
MAPK cascade
cell adhesion
axon guidance
positive regulation of GTPase activity
viral entry into host cell
regulation of synaptic plasticity
interferon-gamma-mediated signaling pathway
regulation of exocyst assembly
cell surface receptor signaling pathway
aging
learning or memory
response to lead ion
response to fluoxetine
thalamus development
neuron projection development
multicellular organismal response to stress
homotypic cell-cell adhesion
response to cocaine
neuron development
positive regulation of calcium-mediated signaling
regulation of sensory perception of pain
positive regulation of cardiac muscle cell proliferation
negative regulation of cell death
response to inorganic substance
peripheral nervous system axon regeneration
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
organ regeneration
cellular response to molecule of bacterial origin
cellular response to inorganic substance
