Invitrogen

Phospho-Ezrin/Radixin/Moesin (Thr567, Thr564, Thr558) Recombinant Rabbit Monoclonal Antibody (PSH05-73)

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说明书
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Phospho-Ezrin/Radixin/Moesin (Thr567, Thr564, Thr558) Recombinant Rabbit Monoclonal Antibody (PSH05-73)

产品信息

MA5-56581

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:2,000
-

免疫组化（石蜡） (IHC (P))

1:1,000
-

免疫细胞化学 (ICC/IF)

1:100
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH05-73

抗原

Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Dry ice (international)

RRID

AB_3679607

产品详细信息

Positive Control: HeLa starved for 3 hours then add 100 nM Calyculin A for 30 minutes cell lysate, NIH/3T3 starved for 24 hours then add 100 nM Calyculin A for 30 minutes cell lysate, C6 treated with 100 ng/mL Calyculin A for 1 hours cell lysate, mouse liver tissue, rat liver tissue.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cell membrane. Cell projection. Cytoplasm. Cytoskeleton. Membrane.

Sequence Similarities: 100% Mouse/Rat.

Predicted band size: 69 kDa.

靶标信息

Moesin, a member of the talin-4.1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cytovillin; cytovillin 2; epididymis luminal protein 70; epididymis secretory protein Li 105; ESP10; Ezrin; Membrane-organizing extension spike protein; Moesin; p81; Radixin; radixin isoform; villin 2; villin 2 (ezrin); Villin-2

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基因别名: AA516625; AW146364; C78546; CVIL; CVL; DFNB24; EZR; HEL-S-105; HEL70; MSN; p81; R75297; RDX; VIL2

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UniProt ID: (Human) P15311, (Mouse) P26040, (Rat) P31977, (Human) P26038, (Rat) O35763, (Mouse) P26041, (Human) P35241, (Mouse) P26043

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Entrez Gene ID: (Human) 7430, (Mouse) 22350, (Rat) 54319, (Human) 4478, (Rat) 81521, (Mouse) 17698, (Human) 5962, (Rat) 315655, (Mouse) 19684

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功能
actin binding protein binding microtubule binding protein domain specific binding protein kinase A catalytic subunit binding protein kinase A regulatory subunit binding S100 protein binding poly(A) RNA binding cell adhesion molecule binding actin filament binding ATPase binding cadherin binding involved in cell-cell adhesion cytoskeletal protein binding protein complex binding structural molecule activity double-stranded RNA binding receptor binding structural constituent of cytoskeleton protein kinase binding protein binding, bridging protein homodimerization activity protein kinase A binding actin or actin-binding cytoskeletal protein
参与通路
negative regulation of transcription from RNA polymerase II promoter intestinal D-glucose absorption sphingosine-1-phosphate signaling pathway cytoskeletal anchoring at plasma membrane leukocyte cell-cell adhesion axon guidance regulation of cell shape regulation of cell size positive regulation of gene expression protein kinase A signaling gland morphogenesis membrane to membrane docking microvillus assembly astral microtubule organization actin cytoskeleton reorganization receptor internalization regulation of microvillus length regulation of actin cytoskeleton organization cellular protein complex localization positive regulation of multicellular organism growth cortical microtubule organization establishment of epithelial cell apical/basal polarity filopodium assembly phosphatidylinositol-mediated signaling positive regulation of protein secretion negative regulation of T cell receptor signaling pathway actin filament bundle assembly establishment of centrosome localization establishment of endothelial barrier negative regulation of ERK1 and ERK2 cascade cellular response to cAMP protein localization to cell cortex establishment of protein localization to plasma membrane positive regulation of establishment of protein localization to plasma membrane negative regulation of interleukin-2 secretion regulation of NIK/NF-kappaB signaling regulation of organelle assembly terminal web assembly positive regulation of protein localization to early endosome positive regulation of cellular protein catabolic process negative regulation of p38MAPK cascade positive regulation of early endosome to late endosome transport establishment or maintenance of apical/basal cell polarity epithelial cell differentiation movement of cell or subcellular component cytoskeleton organization leukocyte migration cellular response to testosterone stimulus positive regulation of podosome assembly regulation of lymphocyte migration positive regulation of cell migration regulation of actin filament bundle assembly regulation of Rap protein signal transduction negative regulation of homotypic cell-cell adhesion negative regulation of GTPase activity regulation of GTPase activity apical protein localization establishment of protein localization negative regulation of cell size actin filament capping cellular response to thyroid hormone stimulus cell-cell adhesion regulation of ruffle assembly positive regulation of G1/S transition of mitotic cell cycle negative regulation of adherens junction organization cellular response to platelet-derived growth factor stimulus barbed-end actin filament capping
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