Invitrogen

Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)

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62 篇参考文献
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Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)

产品信息

44-625G

应用
建议稀释比
已发表文章

免疫印迹 (WB)

Assay-dependent
查看39篇已发表文章 39篇已发表文章

免疫组化 (IHC)

-
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免疫组化（冰冻） (IHC (F))

-
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免疫细胞化学 (ICC/IF)

Assay-dependent
查看10篇已发表文章 10篇已发表文章

免疫沉淀 (IP)

-
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其他PubMed (Misc)

-
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产品规格

种属反应

Chicken, Fruit fly, Human, Mouse, Rat, Xenopus

已发表种属

Fish, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

141-9

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533702

产品详细信息

This antibody will cross-react with the corresponding autophosphorylation site on Proline-rich/Ca2+- activated tyrosine kinase (Pyk2), [pY402]. FAK [pY397] polyclonal antibody (Cat. No. 44-624G) does not cross-react with Pyk2.

The antibody has been used in western blotting. Previous lots of this antibody have been used in immunocytochemistry. For western blotting applications, we recommend using the antibody at a 1:1,000 starting dilution. For immunofluorecence staining applications, we recommend a 1:50 starting dilution. Positive control used: Primary chicken embryo fibroblasts expressing human FAK and plated on fibronectin.

We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20° C. For shipment or short-term storage (up to one week), 2-8° C is sufficient. Expires one year from date of receipt when stored as instructed.

靶标信息

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; EC 2.7.10.2; FADK 1; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase

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基因别名: CG10023; CT28129; DFAK; DFak56; Dmel\CG10023; Dmel_CG10023; DmFAK; FADK; FAK; FAK1; Fak56; Fak56D; FAK65D; FRNK; Kiaa4203; mKIAA4203; p125FAK; pFAK; pp125FAK; PPP1R71; PTK2; XELAEV_18032408mg; XPFAK

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UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346, (Xenopus) Q91738

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Entrez Gene ID: (Fruit fly) 37233, (Human) 5747, (Chicken) 396416, (Mouse) 14083, (Rat) 25614, (Xenopus) 399286

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功能
non-membrane spanning protein tyrosine kinase activity ATP binding actin binding protein kinase activity protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding JUN kinase binding protein kinase binding SH2 domain binding protease binding calcium-dependent cysteine-type endopeptidase activity identical protein binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding
参与通路
MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development heart morphogenesis cellular component disassembly involved in execution phase of apoptosis axon guidance embryo development regulation of endothelial cell migration establishment of cell polarity netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity vascular endothelial growth factor receptor signaling pathway cell motility regulation of cytoskeleton organization cytoskeleton organization response to mechanical stimulus response to glucose response to organic substance response to organonitrogen compound response to organic cyclic compound response to estradiol response to drug fat cell differentiation response to arsenic-containing substance proteolysis actin filament organization transmembrane receptor protein tyrosine kinase signaling pathway response to pH negative regulation of cell-substrate adhesion pyramidal neuron migration cell differentiation negative regulation of protein autophosphorylation positive regulation of protein binding response to muscle stretch wound healing, spreading of cells positive regulation of focal adhesion assembly angiogenesis involved in wound healing positive regulation of protein tyrosine kinase activity positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of receptor recycling negative regulation of synaptic growth at neuromuscular junction
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