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Invitrogen
Phospho-JNK1/JNK2 (Thr183, Tyr185) Recombinant Rabbit Monoclonal Antibody (D12H7L17)
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Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (700031) in ICC/IF
Immunofluorescent analysis of Phospho-JNK1/JNK2 pThr183+pTyr185 in HeLa cells using a Phospho-JNK1/JNK2 pThr183+pTyr185 recombinant rabbit monoclonal antibody (Product # 700031) at a dilution of 5 µg/mL in the absence of peptide (left) or in the presence of the immunogenic peptide (right), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000.
产品信息
700031
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Insect,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
D12H7L17
抗原
A peptide corresponding to amino acids 179-189 and 180-189 of P45983 and P45984, respectively.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with mouse, rat, primate, canine, bovine, porcine, chicken, equine, zebrafish and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; JNK 55; JNK-46; JNK-55; JNK/SAPK alpha; JNK1 beta1 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; Mitogen-activated protein kinase 9; OTTHUMP00000019552; Phospho-JNK; PRKM; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK 1; SAPK1a; SAPK1c; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2
基因别名: AI849689; AI851083; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK8; MAPK9; p54a; p54aSAPK; PRKM8; PRKM9; SAPK; SAPK1; SAPK1A; SAPK1C
UniProt ID: (Human) P45983, (Human) Q15711, (Mouse) Q91Y86, (Mouse) Q9WTU6
Entrez Gene ID: (Human) 5599, (Human) 5601, (Mouse) 26419, (Mouse) 26420
protein serine/threonine kinase activity
JUN kinase activity
protein binding
ATP binding
kinase activity
enzyme binding
histone deacetylase regulator activity
histone deacetylase binding
transcription factor binding
cysteine-type endopeptidase activator activity involved in apoptotic process
mitogen-activated protein kinase kinase kinase binding
nucleotide binding
protein kinase activity
MAP kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
response to stress
JNK cascade
JUN phosphorylation
response to UV
positive regulation of gene expression
regulation of macroautophagy
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
regulation of histone deacetylation
positive regulation of cyclase activity
negative regulation of protein binding
regulation of protein localization
Fc-epsilon receptor signaling pathway
regulation of circadian rhythm
positive regulation of apoptotic process
negative regulation of apoptotic process
rhythmic process
regulation of sequence-specific DNA binding transcription factor activity
positive regulation of protein metabolic process
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
positive regulation of deacetylase activity
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
release of cytochrome c from mitochondria
positive regulation of protein phosphorylation
protein targeting to mitochondrion
activation of cysteine-type endopeptidase activity involved in apoptotic process
central nervous system development
response to mechanical stimulus
response to toxic substance
positive regulation of macrophage derived foam cell differentiation
positive regulation of cell morphogenesis involved in differentiation
response to amine
neuron projection development
positive regulation of prostaglandin biosynthetic process
regulation of protein ubiquitination
positive regulation of prostaglandin secretion
positive regulation of chemokine production
cellular response to UV
response to drug
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-templated
regulation of JNK cascade
response to cadmium ion
positive regulation of nitric-oxide synthase biosynthetic process
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
positive regulation of podosome assembly
positive regulation of apoptotic signaling pathway
ossification
regulation of transcription, DNA-templated
inflammatory response
response to osmotic stress
signal transduction
response to heat
regulation of gene expression
phosphorylation
positive regulation of cell migration
positive regulation of microtubule polymerization
response to hydrogen peroxide
positive regulation of DNA replication
determination of dorsal identity
cellular response to hydrogen peroxide
cellular response to nitric oxide
type B pancreatic cell apoptotic process
neuronal stem cell population maintenance
programmed necrotic cell death
positive regulation of determination of dorsal identity
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
