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Invitrogen
Phospho-MKP1/MKP2 (Ser296, Ser318) Polyclonal Antibody
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Phospho-MKP1/MKP2 (Ser296, Ser318) Antibody (PA5-104854) in ICC/IF
Immunofluorescent analysis of Phospho-MKP1/MKP2 (Ser296, Ser318) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MKP1/MKP2 (Ser296, Ser318) polyclonal antibody (Product # PA5-104854) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-r... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-104854
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DUSP1(Accession P28562), corresponding to amino acid residues around phosphorylated Ser296.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of MKP1/2 only when phosphorylated at Serine 296/318.
靶标信息
There are ten mitogen-activated protein kinase (MAPK) phosphatases (MKPs) that act as negative regulators of MAPK activity in mammalian cells. MKP2 (DUSP4) is a dual threonine/tyrosine phosphatase that specifically dephosphorylates and inactivates MAPKs (ERK, JNK, p38). It contains one Rhodanese domain and one tyrosine-protein phosphatase domain. MKP2 is expressed in a variety of tissues, and is localized in the nucleus. Transcription factor E2F-1 acts as a transcriptional regulator of MKP2. E2F-1/MKP2 pathway mediates apoptosis under oxidative stress and that MKP2 suppresses tumor formation. MKP2 is a novel transcription target of p53 in mediating apoptosis by 10-bp palindrome motif (CTGGCGCCAG) in the MKP2 promoter.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 3CH134/CL100 PTPase; CL 100; dual specificity protein phosphatase hVH1; dual specificity protein phosphatase hVH2; DUSP 4; DUSP-4; MAP kinase phosphatase 1; MAP kinase phosphatase 2; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; mitogen-activated protein kinase phosphatase 1; mitogen-activated protein kinase phosphatase 2; mitogen-activated protein kinase phosphatase-1; MKP II; MKPII; oxidative stress-inducible protein tyrosine phosphatase; protein tyrosine phosphatase, non-receptor type 16; protein-tyrosine phosphatase 3CH134; protein-tyrosine phosphatase CL100; protein-tyrosine phosphatase ERP; protein-tyrosine phosphatase non-receptor type 16; serine/threonine specific protein phosphatase; unnamed protein product; VH1 homologous phosphatase 2
基因别名: 2700078F24Rik; 3CH134; CL100; E130306H24Rik; erp; HVH1; HVH2; MKP-1; MKP-2; MKP1; MKP2; PTPN10; Ptpn16; TYP
UniProt ID: (Human) P28562, (Human) Q13115, (Rat) Q64623, (Mouse) P28563, (Mouse) Q8BFV3, (Rat) Q62767
Entrez Gene ID: (Human) 1843, (Human) 1846, (Rat) 114856, (Mouse) 19252, (Mouse) 319520, (Rat) 60587
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
protein tyrosine phosphatase activity
protein binding
protein tyrosine/serine/threonine phosphatase activity
MAP kinase tyrosine/serine/threonine phosphatase activity
growth factor binding
mitogen-activated protein kinase binding
protein tyrosine/threonine phosphatase activity
phosphatase activity
MAP kinase phosphatase activity
MAP kinase threonine phosphatase activity
endoderm formation
protein dephosphorylation
negative regulation of cell adhesion
signal transduction
negative regulation of cell proliferation
response to light stimulus
response to estradiol
response to retinoic acid
cellular response to hormone stimulus
response to testosterone
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
peptidyl-threonine dephosphorylation
response to hydrogen peroxide
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAPK cascade
response to glucocorticoid
negative regulation of meiotic cell cycle
response to cAMP
response to calcium ion
peptidyl-serine dephosphorylation
negative regulation of ERK1 and ERK2 cascade
negative regulation of monocyte chemotaxis
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of p38MAPK cascade
cellular response to chemokine
negative regulation of DNA biosynthetic process
ERK1 and ERK2 cascade
dephosphorylation
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