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Invitrogen
Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Polyclonal Antibody
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FIGURE: 1 / 17
Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody (44-888G) in WB
Peptide Competition: Extracts prepared from 293T cells left unstimulated (1) or stimulated with HGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C
Product Details
44-888G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Dog,
Hamster,
Human,
Mouse,
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. The sequence is conserved in mouse and rat.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
44-888G has been used successfully in peptide-ELISA. Dilution is to be determined by the consumer.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met
Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233, (Mouse) 17295
protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
ATP binding
protein phosphatase binding
nucleotide binding
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
beta-catenin binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
phospholipase binding
phosphatidylinositol 3-kinase binding
protein heterodimerization activity
transmembrane signal receptor
endothelial cell morphogenesis
signal transduction
cell surface receptor signaling pathway
cell proliferation
negative regulation of autophagy
peptidyl-tyrosine phosphorylation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
branching morphogenesis of an epithelial tube
positive chemotaxis
semaphorin-plexin signaling pathway
negative regulation of hydrogen peroxide-mediated programmed cell death
positive regulation of endothelial cell chemotaxis
negative regulation of transcription from RNA polymerase II promoter
activation of MAPK activity
neuron migration
liver development
placenta development
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
brain development
muscle organ development
skeletal muscle tissue development
positive regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
negative regulation of peptidyl-threonine phosphorylation
positive regulation of glucose transport
positive regulation of neuron projection development
muscle cell migration
myotube differentiation
phosphorylation
sperm motility
adult behavior
positive regulation of peptidyl-serine phosphorylation
glucose homeostasis
positive regulation of DNA replication
positive regulation of mitotic nuclear division
protein autophosphorylation
positive regulation of dendrite morphogenesis
modulation of synaptic transmission
myoblast proliferation
excitatory postsynaptic potential
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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