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Invitrogen
Phospholamban Monoclonal Antibody (2D12)
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Phospholamban Antibody (MA3-922) in ICC/IF
Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% B... View More
产品信息
MA3-922
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Chicken,
Guinea pig,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Rat
已发表种属
C. elegans,
Dog,
Guinea pig,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Rodent
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
2D12
抗原
Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine Phospholamban.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse.
MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues.
The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.
靶标信息
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cardiac CMH18; cardiac phospholamban; Phospholamban; PLB; unnamed protein product
基因别名: CMD1P; CMH18; PLB; Plm; PLN
UniProt ID: (Chicken) P26677, (Rabbit) P61015, (Human) P26678, (Pig) P61013, (Dog) P61012, (Rat) P61016, (Mouse) P61014
regulation of the force of heart contraction
calcium ion transport
cellular calcium ion homeostasis
regulation of heart contraction
response to zinc ion
negative regulation of heart rate
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
negative regulation of ATPase activity
response to insulin
response to testosterone
negative regulation of heart contraction
cardiac muscle tissue development
protein homooligomerization
regulation of calcium ion transport
negative regulation of calcium ion transport
regulation of ryanodine-sensitive calcium-release channel activity
regulation of cardiac muscle cell contraction
adrenergic receptor signaling pathway involved in heart process
regulation of the force of heart contraction by cardiac conduction
regulation of calcium ion import
negative regulation of calcium ion transmembrane transporter activity
regulation of relaxation of muscle
negative regulation of calcium ion binding
regulation of calcium-transporting ATPase activity
negative regulation of calcium-transporting ATPase activity
negative regulation of catalytic activity
Notch signaling pathway
blood circulation
regulation of cytosolic calcium ion concentration
relaxation of cardiac muscle
regulation of cardiac muscle cell membrane potential
negative regulation of calcium ion import
regulation of relaxation of cardiac muscle
negative regulation of calcium ion import into sarcoplasmic reticulum
regulation of cardiac conduction
