Invitrogen

Progesterone Receptor Monoclonal Antibody (PR-AT 4.14)

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56 篇参考文献
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Progesterone Receptor Monoclonal Antibody (PR-AT 4.14)

产品信息

MA1-410

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:200-1:1,000
查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

-
查看33篇已发表文章 33篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL
查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

5 µg/mL
-

免疫细胞化学 (ICC/IF)

-
查看10篇已发表文章 10篇已发表文章

免疫沉淀 (IP)

Assay-dependent
查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀 实验 (ChIP)

Assay-dependent
-
产品规格

种属反应

Bovine, Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

Bovine, C. elegans, Dog, Guinea pig, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

PR-AT 4.14

抗原

Synthetic peptide corresponding to residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.
查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2164327

产品详细信息

MA1-410 detects the progesterone receptor (PR) from human, non-human primate, bovine, rat, rabbit, and mouse tissues. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor.

MA1-410 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from T47D whole cell lysate.

MA1-410 performs better by western blot using BSA as the blocking agent and in antibody diluent.

The MA1-410 immunogen is a synthetic peptide corresponding to the binding domain residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.

MA1-410 can be used with blocking peptide PEP-016.

靶标信息

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor; progesterone-receptor; steroid hormone receptor

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基因别名: 9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B

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UniProt ID: (Rabbit) P06186, (Human) P06401, (Rat) Q63449, (Mouse) Q00175

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Entrez Gene ID: (Rabbit) 100009094, (Bovine) 100848466, (Human) 5241, (Rat) 25154, (Mouse) 18667

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding enzyme binding ATPase binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding hormone binding sequence-specific DNA binding lipid binding metal ion binding
参与通路
ovulation from ovarian follicle luteinization epithelial cell maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of gene expression positive regulation of smooth muscle cell migration diterpenoid metabolic process intracellular receptor signaling pathway mammary gland development response to progesterone response to cocaine negative regulation of apoptotic process steroid hormone mediated signaling pathway response to estrogen estrous cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of epithelial cell proliferation progesterone receptor signaling pathway female mating behavior tertiary branching involved in mammary gland duct morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus cellular response to luteinizing hormone stimulus transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling paracrine signaling lung alveolus development
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