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Invitrogen
RAD51 Monoclonal Antibody (14B4)
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FIGURE: 1 / 15
RAD51 Antibody (MA1-23271) in ICC/IF
Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (Product # MA1-23271). Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1,000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.
Product Details
MA1-23271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
C. elegans,
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
14B4
Immunogen
Full length (amino acids 1-338) Rad51 expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control for this product is T24.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603
UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297
Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361, (Rat) 499870
recombinase activity
four-way junction DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
ATP binding
protein C-terminus binding
identical protein binding
single-stranded DNA-dependent ATPase activity
DNA polymerase binding
DNA-dependent ATPase activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
nucleotide binding
DNA binding
damaged DNA binding
molecular_function
DNA metabolism protein
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA synthesis involved in DNA repair
strand displacement
regulation of protein phosphorylation
DNA unwinding involved in DNA replication
DNA repair
DNA recombination
mitotic recombination
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
response to toxic substance
response to X-ray
regulation of double-strand break repair via homologous recombination
telomere maintenance via telomere lengthening
replication fork processing
strand invasion
response to drug
positive regulation of DNA ligation
protein homooligomerization
chromosome organization involved in meiotic cell cycle
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to hydroxyurea
cellular response to cisplatin
cellular response to camptothecin
response to etoposide
replication-born double-strand break repair via sister chromatid exchange
mitotic recombination-dependent replication fork processing
response to ionizing radiation
interstrand cross-link repair
meiotic cell cycle
DNA metabolic process
telomere organization
cellular response to alkaloid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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