Invitrogen

RHOA Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Tech Support
RHOA Polyclonal Antibody

Product Details

PA5-99537

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RHOA(Accession P61586), corresponding to amino acid residues T163-L193.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818470

Product Specific Information

Antibody detects endogenous levels of total RhoA.

Target Information

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aplysia ras-related homolog 12; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; h12; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; Rho; Rho cDNA clone 12; Rho family GTPase; small GTP binding protein RhoA; Transforming protein RhoA

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Gene Aliases: ARH12; ARHA; Arha1; Arha2; RHO12; RHOA; RHOH12

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UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0

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Entrez Gene ID: (Human) 387, (Rat) 117273, (Mouse) 11848

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Function(s)
GTPase activity protein binding GTP binding myosin binding GDP binding protein domain specific binding Rho GDP-dissociation inhibitor binding nucleotide binding small GTPase
Process(es)
transforming growth factor beta receptor signaling pathway Rho protein signal transduction viral process cell migration substantia nigra development actin cytoskeleton organization platelet activation regulation of cell migration actin cytoskeleton reorganization positive regulation of cytokinesis regulation of actin cytoskeleton organization regulation of osteoblast proliferation Roundabout signaling pathway cleavage furrow formation apolipoprotein A-I-mediated signaling pathway positive regulation of NF-kappaB import into nucleus positive regulation of I-kappaB kinase/NF-kappaB signaling stress fiber assembly apical junction assembly endothelial cell migration ossification involved in bone maturation wound healing, spreading of cells positive regulation of neuron differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling negative regulation of axonogenesis positive regulation of axonogenesis negative chemotaxis regulation of small GTPase mediated signal transduction positive regulation of stress fiber assembly Wnt signaling pathway, planar cell polarity pathway positive regulation of lipase activity trabecula morphogenesis positive regulation of protein serine/threonine kinase activity negative regulation of cell migration involved in sprouting angiogenesis mitotic spindle assembly endothelial tube lumen extension skeletal muscle satellite cell migration mitotic cleavage furrow formation regulation of cell motility cell morphogenesis response to hypoxia regulation of transcription from RNA polymerase II promoter cytoskeleton organization cell adhesion cell-matrix adhesion small GTPase mediated signal transduction skeletal muscle tissue development regulation of actin polymerization or depolymerization metabolic process response to mechanical stimulus response to glucose regulation of neuron projection development cerebral cortex cell migration forebrain radial glial cell differentiation cell differentiation positive regulation of cell growth positive regulation of cell migration androgen receptor signaling pathway positive regulation of actin filament polymerization stress-activated protein kinase signaling cascade negative regulation of intracellular steroid hormone receptor signaling pathway response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to ethanol negative regulation of neuron differentiation positive regulation of translation positive regulation of cell adhesion positive regulation of vasoconstriction positive regulation of smooth muscle contraction neuron projection morphogenesis regulation of dendrite development response to glucocorticoid regulation of calcium ion transport negative regulation of cell death cellular response to progesterone stimulus positive regulation of podosome assembly regulation of neural precursor cell proliferation cell cycle integrin-mediated signaling pathway GTP metabolic process cell division
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