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Invitrogen
RIAM Polyclonal Antibody
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FIGURE: 1 / 3
RIAM Antibody (PA5-114857) in ICC/IF
Immunocytochemistry analysis of RIAM in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114857) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human APBB1IP(Accession Q7Z5R6), corresponding to amino acid residues S475-S525.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total APBB1IP.
Target Information
RIAM appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; PREL-1; Proline-rich EVH1 ligand 1; Proline-rich protein 48; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; RARP-1; Retinoic acid-responsive proline-rich protein 1; RIAM; RIAMRARP-1
Gene Aliases: 9930118P07Rik; APBB1IP; PREL1; Prp48; RARP1; RIAM
UniProt ID: (Mouse) Q8R5A3
Entrez Gene ID: (Mouse) 54519
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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