Invitrogen

ROBO1 Monoclonal Antibody (10E2)

Name: ROBO1 Monoclonal Antibody (10E2)
Brand: Invitrogen
SKU: MA5-36105

查看其他24个ROBO1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)

ROBO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

ROBO1 Antibody (MA5-36105) in IHC (P)

Immunohistochemical analysis of ROBO1 in paraffin-embedded human stomach cancer tissue using a monoclonal antibody (Product #MA5-36105). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:100) for 30 m... View More

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Immunogen Image ROBO1 Monoclonal Antibody (10E2)

产品信息

MA5-36105

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:50-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

10E2

抗原

Recombinant protein within Human ROBO1 aa 41-286

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2890404

靶标信息

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1

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基因别名: AW494633; AW742721; DUTT1; Gm310; ROBO1; SAX3

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UniProt ID: (Human) Q9Y6N7, (Mouse) O89026

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Entrez Gene ID: (Human) 6091, (Mouse) 19876

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功能

protein binding axon guidance receptor activity LRR domain binding identical protein binding scaffold/adaptor protein

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