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Invitrogen
Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
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Rat IgG2a kappa Isotype Control (12-4321-82) in Flow
Mouse bone marrow cells were cultured in the presence of GM-CSF for 7 days, Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and subsequently stained with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and 0.06 µg of Rat IgG2a kappa Isotype Control, PE (Product # 12-4321-82) (left) or 0.06 µg of Axl Monoclonal Antibody, PE (right). Total viable cells were used for ana... View More
产品信息
12-4321-82
应用
建议稀释比
免疫细胞化学 (ICC/IF)
5 µg/mL
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBR2a
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis.
Applications Tested: This Rat IgG2a K Isotype Control has been tested by flow cytometric analysis of mouse splenocytes and should be used at the same concentration as the experimental antibody.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
