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Invitrogen
SERCA2 ATPase Monoclonal Antibody (IID8)
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SERCA2 ATPase Antibody (MA3-910) in ICC/IF
Immunofluorescence analysis of SERCA2 was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SERCA2 ATPase Monoclonal Antibody (Product # MA3-910) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnigh... View More
产品信息
MA3-910
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Human,
Mouse,
Pig,
Rabbit,
Rat
已发表种属
Amphibian,
C. elegans,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Sheep,
Virus,
Zebrafish
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
IID8
抗原
Purified canine cardiac sarcoplasmic reticulum.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-910 detects sarcoplasmic or endoplasmic reticulum calcium 2 (SERCA2) ATPase from bovine, canine, human, pig, rabbit and rat tissues. This antibody does not detect frog SERCA2 ATPase (J. Biol. Chem., Feb 2001; 276: 3911 - 3919). MA3-910 detects SERCA2 ATPase and does not discriminate between the two isoforms.
MA3-910 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle with MA3-910 results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization.
MA3-910 is not recommended for Western blot detection of rat SERCA2.
The MA3-910 antigen is purified canine cardiac sarcoplasmic reticulum.
靶标信息
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; ATPase, Ca++ transporting, slow twitch 2; Ca(2+)-transport ATPase class 3; calcium ATPase; Calcium pump 2; calcium-ATPase (EC 3.6.1.3); Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarco(endo)plasmic reticulum Ca(2+)-dependent ATPase 2; sarco/endoplasmic reticulum Ca2+-ATPase 2; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; sarcoplasmic/endoplasmic-reticulum Ca(2+) pump gene 2; SERCA; SERCA ATPase; SERCA2; SR Ca(2+)-ATPase 2; unnamed protein product
基因别名: 9530097L16Rik; ATP2; ATP2A2; ATP2B; D5Wsu150e; DAR; DD; mKIAA4195; SERCA2; SERCA2A; SERCA2B; SercaII
UniProt ID: (Dog) O46674, (Rabbit) P20647, (Human) P16615, (Pig) P11607, (Mouse) O55143, (Rat) P11507
Entrez Gene ID: (Dog) 403878, (Bovine) 540568, (Rabbit) 100038308, (Human) 488, (Pig) 396875, (Mouse) 11938, (Rat) 29693
ATP binding
metal ion binding
calcium-transporting ATPase activity
calcium ion binding
protein binding
protein C-terminus binding
enzyme binding
lutropin-choriogonadotropic hormone receptor binding
S100 protein binding
calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential
ion channel binding
nucleotide binding
hydrolase activity
primary active transporter
regulation of cardiac muscle cell membrane potential
hydrogen ion transmembrane transport
regulation of the force of heart contraction
ER-nucleus signaling pathway
organelle organization
cell adhesion
epidermis development
positive regulation of heart rate
regulation of cardiac muscle contraction by calcium ion signaling
transition between fast and slow fiber
endoplasmic reticulum calcium ion homeostasis
positive regulation of endoplasmic reticulum calcium ion concentration
T-tubule organization
ion transmembrane transport
cellular response to oxidative stress
response to endoplasmic reticulum stress
response to peptide hormone
negative regulation of heart contraction
relaxation of cardiac muscle
sarcoplasmic reticulum calcium ion transport
calcium ion transmembrane transport
regulation of cardiac muscle cell action potential involved in regulation of contraction
regulation of calcium ion-dependent exocytosis of neurotransmitter
calcium ion transport from cytosol to endoplasmic reticulum
regulation of cardiac conduction
calcium ion import into sarcoplasmic reticulum
cardiac muscle hypertrophy in response to stress
negative regulation of receptor binding
transport
ion transport
calcium ion transport
cellular calcium ion homeostasis
regulation of muscle contraction
metabolic process
