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Invitrogen
SF3B14 Polyclonal Antibody
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FIGURE: 1 / 3
SF3B14 Antibody (PA5-101771) in IHC (P)
Immunohistochemistry analysis of SF3B14 in human ovarian cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SF3B14 polyclonal antibody (Product # PA5-101771) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-101771
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SF3B6(Accession Q9Y3B4), corresponding to amino acid residues Y92-K125.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SF3B14.
Target Information
SF3B14 encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pre-mRNA branch site protein p14; SF3B 14 kDa subunit; spliceosome-associated protein, 14 kDa subunit; spliceosome-associated protein, 14-kDa; splicing factor 3B, 14 kDa subunit; splicing factor 3B, 14 kDa subunit-like; splicing factor 3b, subunit 6, 14kDa; unnamed protein product
Gene Aliases: 0610009D07Rik; 6030419K15Rik; CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a
UniProt ID: (Human) Q9Y3B4, (Mouse) P59708
Entrez Gene ID: (Human) 51639, (Mouse) 66055, (Rat) 100365504
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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