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Invitrogen
SHARPIN Polyclonal Antibody
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FIGURE: 1 / 2
SHARPIN Antibody (PA5-88439) in WB
Western blot analysis of SHARPIN in various lysates. Samples were incubated with SHARPIN Polyclonal antibody (Product # PA5-88439) using a dilution of 1:900, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-88439
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human SHARPIN (NP_1122363?)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.08 mg/mL
Amount
208 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: 293T, LO2, HeLa, A-549, Rat brain; Cellular Location: Cell junction, Cytoplasm, cytosol, synapse
Immunogen sequence: MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS LHFLNPQEAQ RWAVLVRGAT VEGQNG
Target Information
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mSIPL1; protein kinase C-interacting protein RBCC like 1; SHANK-associated RH domain interacting protein; Shank-associated RH domain-interacting protein; shank-interacting protein; Shank-interacting protein-like 1; Sharpin
Gene Aliases: 0610041B22Rik; AW121341; Conneck1; Cpdm; RBCKL1; Sharpin; SIPL1
UniProt ID: (Rat) Q9EQL9, (Mouse) Q91WA6
Entrez Gene ID: (Rat) 81859, (Mouse) 106025
mitochondrion organization
brain development
epidermis development
regulation of tumor necrosis factor-mediated signaling pathway
apoptotic nuclear changes
keratinization
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of inflammatory response
protein homooligomerization
protein linear polyubiquitination
regulation of CD40 signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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