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Rockland
SIP1 Polyclonal Antibody
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FIGURE: 1 / 3
SIP1 Antibody (600-401-EL0) in ICC/IF
Immunocytochemistry of SIP1 antibody. Tissue: HeLa cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SIP1 antibody at 4 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SIP1 is nuclear and cytoplasmic. Staining: SIP1 as precipitated blue signal with pink nuclear counterstain.
Product Details
600-401-EL0
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-SIP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide near the C-terminus of human SIP1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; Zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b
Gene Aliases: 9130203F04Rik; D130016B08Rik; HRIHFB2411; HSPC082; KIAA0569; SIP-1; SIP1; SMADIP1; ZEB2; ZFHX1B; ZFX1B; Zfxh1b
UniProt ID: (Human) O60315, (Mouse) Q9R0G7
Entrez Gene ID: (Human) 9839, (Mouse) 24136, (Rat) 311071
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
phosphatase regulator activity
metal ion binding
R-SMAD binding
nucleic acid binding
molecular_function
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
neural crest cell migration
somitogenesis
neural tube closure
transcription from RNA polymerase II promoter
nervous system development
corpus callosum morphogenesis
hippocampus development
cell proliferation in forebrain
corticospinal tract morphogenesis
positive regulation of Wnt signaling pathway
positive regulation of JUN kinase activity
positive regulation of melanocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of melanin biosynthetic process
developmental pigmentation
collateral sprouting
positive regulation of axonogenesis
mammillary axonal complex development
melanocyte migration
positive regulation of lens fiber cell differentiation
regulation of melanosome organization
transcription, DNA-templated
regulation of transcription, DNA-templated
central nervous system development
embryonic morphogenesis
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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