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Invitrogen
SKP2 Recombinant Rabbit Monoclonal Antibody (2L0K1)
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SKP2 Antibody (MA5-35433) in ICC/IF
Immunofluorescence analysis of SKP2 was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SKP2 Recombinant Rabbit Monoclonal Antibody (ARC0886) (Product # MA5-35433) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
产品信息
MA5-35433
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
2L0K1
抗原
Synthetic peptide
偶联物
形式
Liquid
浓度
0.8 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MHRKHLQEIP DLSSNVATSF TWGWDSSKTS ELLSGMGVSA LEKEEPDSEN IPQELLSNLG HPESPPRKRL KSKGSDKDFV IVRRPKLNRE NFPGVSWDSL
靶标信息
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; F-box/WD-40 protein 1; FWD1; MGC1366; OTTHUMP00000221389; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 (p45)
基因别名: 4930500A04Rik; FBL1; FBXL1; FLB1; FWD1; p45; RGD1562456; SKP2
UniProt ID: (Human) Q13309, (Mouse) Q9Z0Z3
Entrez Gene ID: (Human) 6502, (Rat) 294790, (Mouse) 27401
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
protein polyubiquitination
ubiquitin-dependent protein catabolic process
phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
regulation of apoptotic process
positive regulation of smooth muscle cell proliferation
regulation of cell cycle
cellular response to cell-matrix adhesion
positive regulation of protein polyubiquitination
cell proliferation
anaphase-promoting complex-dependent catabolic process
