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Invitrogen
SMG1 Polyclonal Antibody
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FIGURE: 1 / 5
SMG1 Antibody (PA5-115933) in ICC/IF
Immunocytochemistry analysis of SMG1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115933). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SMG1(Accession Q96Q15), corresponding to amino acid residues Q3603-L3653.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Smg1.
Target Information
This gene encodes a protein involved in nonsense-mediated mRNA decay as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: contains FRAP/ATM-like kinase domain; hSMG-1; KIAA0421; lambda-interacting protein; lambda/iota protein kinase C-interacting protein; nonsense mediated mRNA decay-associated PI3K-related kinase SMG1; phosphatidylinositol 3-kinase-related protein kinase; PI-3-kinase-related kinase SMG-1; SMG-1; smg-1 homolog, phosphatidylinositol 3-kinase-related kinase; SMG1 homolog, phosphatidylinositol 3-kinase-related kinase; SMG1 phosphatidylinositol 3-kinase-related kinase; unnamed protein product
Gene Aliases: 2610207I05Rik; 5430435M13Rik; 61E3.4; ATX; C130002K18Rik; LIP; mKIAA0421; RGD1563508
UniProt ID: (Human) Q96Q15, (Mouse) Q8BKX6
Entrez Gene ID: (Human) 23049, (Mouse) 233789, (Rat) 691397
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular response to DNA damage stimulus
peptidyl-serine phosphorylation
regulation of telomere maintenance
protein autophosphorylation
phosphatidylinositol phosphorylation
mRNA export from nucleus
regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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