Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 68
SOX2 Antibody (14-9811-82) in ICC/IF
Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Monoclonal Antibody (Btjce), eBioscience™ (Product # 14-9811-82) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus... View More
产品信息
14-9811-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Bovine,
C. elegans,
Chicken,
Guinea pig,
Human,
Mouse,
Non-human primate
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
Btjce
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).
Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.
Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunocytochemistry, western blot and ELISA (Fluorochrome-conjugated Btjce is recommended for use in intracellular flow cytometry).
Applications Tested: This Btjce antibody has been tested by immunohistochemistry on FFPE human testes at less than or equal to 5 µg/mL. The antibody can be used with either high or low pH antigen retrieval. This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized iPS cells at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Bacterial expression of region after aa135 (homology between species but not to other family members)
靶标信息
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2
基因别名: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
chromatin binding
transcription factor binding
chromatin DNA binding
protein heterodimerization activity
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
eye development
endodermal cell fate specification
chromatin organization
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle arrest
response to wounding
regulation of gene expression
glial cell fate commitment
pituitary gland development
adenohypophysis development
positive regulation of cell-cell adhesion
forebrain development
somatic stem cell population maintenance
tissue regeneration
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
negative regulation of epithelial cell proliferation
response to growth factor
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
cell fate specification
positive regulation of neuroblast proliferation
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
multicellular organism development
sensory perception of sound
response to organic substance
stem cell population maintenance
forebrain neuron differentiation
cerebral cortex development
negative regulation of Wnt signaling pathway
male genitalia development
positive regulation of epithelial cell differentiation
olfactory placode formation
response to retinoic acid
inner ear morphogenesis
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
