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Invitrogen
SPOP Polyclonal Antibody
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SPOP Antibody (PA5-116544) in WB
Western blot analysis of SPOP in extracts of various cell lines. Samples were incubated with SPOP Polyclonal antibody (Product # PA5-116544) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-116544
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 220-374 of human SPOP (NP_0010072271)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
3.78 mg/mL
规格
378 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: HeLa, HT-29
Immunogen sequence: ARSPVFSAMF EHEMEESKKN RVEINDVEPE VFKEMMCFIY TGKAPNLDKM ADDLLAAADK YALERLKVMC EDALCSNLSV ENAAEILILA DLHSADQLKT QAVDFINYHA SDVLETSGWK SMVVSHPHLV AEAYRSLASA QCPFLGPPRK RLKQS
靶标信息
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HIB homolog 1; Roadkill homolog 1; Speckle-type POZ protein
基因别名: SPOP
Entrez Gene ID: (Human) 8405
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