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Invitrogen
SPTLC1 Polyclonal Antibody
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FIGURE: 1 / 5
SPTLC1 Antibody (PA5-95343) in ICC/IF
Immunofluorescence analysis of SPTLC1 in U20S cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with SPTLC1 polyclonal antibody (Product # PA5-95343) at a 2 µg/mL dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95343
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SPTLC1 (444-473aa IRVVVTVEQTEEELERAASTIKEVAQAVLL).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hacat whole cell, human A431 whole cell, human SIHA whole cell, human U251 whole cell. IHC: human intestinal cancer tissue. ICC/IF: U20S cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hLCB1; HSAN; long chain base biosynthesis protein 1; MGC14645; RP11-118F2.1; serine C-palmitoyltransferase; serine-palmitoyl-CoA transferase 1; unnamed protein product
Gene Aliases: ALS27; HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI
UniProt ID: (Human) O15269
Entrez Gene ID: (Human) 10558
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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