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Invitrogen
SRP72 Polyclonal Antibody
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SRP72 Antibody (PA5-143946) in ICC/IF
Immunocytochemistry analysis of SRP72 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SRP72 Polyclonal antibody (Product # PA5-143946) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
产品信息
PA5-143946
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human SRP72 recombinant protein (Position: I40-Y460).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human HL-60 whole cell, rat pancreas tissue, mouse pamcreas tissue. IHC: human breast cancer tissue, human renal cancer tissue, human thyroid cancer tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: U20S cell. Flow: U937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: epididymis luminal protein 103; signal recognition particle 72 kDa; Signal recognition particle 72 kDa protein; signal recognition particle 72kD; signal recognition particle 72kDa; Signal recognition particle subunit SRP72; SRP72
基因别名: 5730576P14Rik; 72kDa; AI132477; BC019196; BMFF; BMFS1; C77589; HEL103; SRP72
UniProt ID: (Human) O76094
Entrez Gene ID: (Human) 6731, (Rat) 498351, (Mouse) 66661
