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Sigma-1 Receptor Antibody (42-3300) in WB
Western blot of SIGMAR1 was performed by loading 30 µg of WT (lanes 1/3) and SIGMAR1 CRISPR KO (lanes 2/4) HEK293T or HeLa cell lysates in RIPA buffer onto a 5-16% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. SIGMAR1 was detected at approximately 25 kDa (designated by the black arrow) using a SIGMAR1 polyclonal antibody (Product # 42-3300) at a dilution of 1:5,000 in 5% BSA in TBS with 0.1% T... View More
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.jpg?time=20220826?time=20220826 "Sigma-1 Receptor Antibody (42-3300) in WB")
产品信息
42-3300
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
C. elegans,
Hamster,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from the C-terminal region of the rat sigma-1 receptor, which differs from mouse and human by one conservative amino acid change and is predicted to be identical to dog, bovine, and ermine
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific for the sigma-1 receptor (sigma-1R, Oprs1 protein, opioid receptor sigma 1) protein. On western blots, it identifies the target band at ~25 kDa. Reactivity has been confirmed with rat brain, rat liver, and mouse brain homogenates. Based on amino acid sequence identity, reactivity with human, bovine, dog, and ermine is expected.
靶标信息
This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AL024364; mSigmaR1; opioid receptor, sigma 1; RP23 167I12.6; SIG 1R; sigma 1 receptor variant SR-1D; Sigma 1-type opioid receptor; Sigma non-opioid intracellular receptor 1; Sigma-1 Receptor; sigma1 receptor; Sigma1-receptor; sigma1R; SR BP
基因别名: Oprs1; sigma1R; Sigmar1
UniProt ID: (Rat) Q9R0C9, (Mouse) O55242
Entrez Gene ID: (Rat) 29336, (Mouse) 18391
