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Invitrogen
Survivin Recombinant Rabbit Monoclonal Antibody (SP07-06)
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Survivin Antibody (MA5-42420) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of Survivin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Survivin Recombinant Monoclonal Antibody (Product # MA5-42420) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (b... View More
产品信息
MA5-42420
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SP07-06
抗原
Synthetic peptide within N-terminal human Survivin
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: Hela, human tonsil tissue, mouse testis tissue, human lymph node tissue.
Subcellular Location: Cytoplasm, Nucleus, Chromosome, Midbody.
靶标信息
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; survivin; survivin variant 3 alpha; SVV; TIAP
基因别名: AAC-11; API4; BIRC5; EPR-1; IAP4; survivin40; TIAP
UniProt ID: (Human) O15392, (Mouse) O70201
Entrez Gene ID: (Human) 332, (Mouse) 11799
ubiquitin-protein transferase activity
cysteine-type endopeptidase inhibitor activity
protein binding
microtubule binding
Ran GTPase binding
enzyme binding
identical protein binding
metal ion binding
chaperone binding
zinc ion binding
tubulin binding
peptidase inhibitor activity
protein homodimerization activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
protein heterodimerization activity
cofactor binding
transcription, DNA-templated
protein phosphorylation
apoptotic process
sister chromatid cohesion
positive regulation of cell proliferation
regulation of signal transduction
protein ubiquitination
protein sumoylation
protein complex localization
regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
cell division
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
G2/M transition of mitotic cell cycle
microtubule cytoskeleton organization
cytokinesis
regulation of transcription, DNA-templated
cell cycle
chromosome segregation
mitotic nuclear division
regulation of mitotic cell cycle
embryo development
negative regulation of peptidase activity
positive regulation of exit from mitosis
spindle checkpoint
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of neuron apoptotic process
positive regulation of cell cycle
positive regulation of mitotic cell cycle
establishment of chromosome localization
regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of type B pancreatic cell proliferation
