Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
SynGAP Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 42
SynGAP Antibody (PA1-046) in ICC/IF
Immunofluorescence analysis of SynGAP was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SynGAP Rabbit Polyclonal Antibody (Product # PA1-046) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
产品信息
PA1-046
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
GST fusion protein of a recombinant fragment, residues 947-1167, of rat SynGAP.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-046 detects synaptic Ras-GTPase activating protein (SynGAP) from mouse and rat tissues.
The PA1-046 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~135 kDa protein representing SynGAP from mouse brain tissue homogenate. Immunocytochemical staining of SynGAP in rat hippocampal neurons with PA1-046 yields a pattern consistent with vesicular staining.
PA1-046 immunogen is residues 947-1167 of rat SynGAP.
靶标信息
Synaptic Ras-GTPase activating protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp761G1421; Neuronal R; Neuronal RasGAP; OTTHUMP00000209046; p135 SynGAP; Ras GTPase-activating protein SynGAP; Ras/Rap GTPase-activating protein SynGAP; synaptic Ras GTPase activating protein 1 homolog; synaptic Ras GTPase activating protein, 135kDa; Synaptic Ras GTPase-activating protein 1; Synaptic Ras-GAP 1
基因别名: Gm1963; KIAA1938; MRD5; RASA1; RASA5; SYNGAP; SYNGAP1
UniProt ID: (Human) Q96PV0, (Mouse) F6SEU4
Entrez Gene ID: (Human) 8831, (Rat) 192117, (Mouse) 240057
MAPK cascade
Ras protein signal transduction
pattern specification process
visual learning
dendrite development
receptor clustering
regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
negative regulation of Ras protein signal transduction
regulation of synaptic plasticity
regulation of long-term neuronal synaptic plasticity
negative regulation of axonogenesis
signal transduction
regulation of synapse structure or activity
regulation of GTPase activity
