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          • Primary Antibodies ›
          • DYKDDDDK Tag Antibodies

          OriGene

          DDK Monoclonal Antibody (OTI4C5 (formerly 4C5)), HRP, TrueMAB™

          1 Reference
          View all (65) DYKDDDDK Tag antibodies

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          Cite DDK Monoclonal Antibody (OTI4C5 (formerly 4C5)), HRP, TrueMAB™

          Additional Information:
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          • Antibody Testing Data (1)
          DDK Antibody in Immunoprecipitation (IP)
          Group 53 Created with Sketch.
          DDK Antibody in Immunoprecipitation (IP)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          DDK Antibody (TA150030) in IP

          Myc/DDK tagged VARS2 overexpression lysate and pull-down by various batches of anti-DDK beads were detected with HRP-conjugated 4C5 anti-DDK antibody (TA150030). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          DDK Antibody in Immunoprecipitation (IP)

          Product Details

          TA150030

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          -

          Immunoprecipitation (IP)

          1:50
          -

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OTI4C5 (formerly 4C5)

          Immunogen

          Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH

          Conjugate

          HRP HRP HRP
          • Unconjugated
          • Agarose
          • Biotin 
          • DyLight 488
          • DyLight 550
          • DyLight 594
          • Magnetic beads
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          50 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 1% BSA, 40% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Synthetic peptide sequence is DYKDDDDK

          Target Information

          DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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