Invitrogen

TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Biotin, eBioscience™

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23 篇参考文献
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问题与答案
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产品信息

13-9924-81

应用
建议稀释比
已发表文章

免疫印迹 (WB)

-
查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

-
查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent
查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

-
查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test
查看12篇已发表文章 12篇已发表文章

中和实验 (Neu)

-
查看2篇已发表文章 2篇已发表文章
产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

推荐的同型对照

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

MTS510

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466991

产品详细信息

Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.

Applications Reported: MTS510 has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.

Applications Tested: The MTS510 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ARMD10; CD284; ESOP-1; hToll; lipopolysaccharide response; Ly-96; Lymphocyte antigen 96; myeloid differentiation factor-2; myeloid differentiation protein-2; Protein MD-2; TLR-4; TOLL; Toll-like receptor 4

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基因别名: ESOP-1; Esop1; Lps; Ly87; Ly96; MD-2; MD2; Ran/M1; Rasl2-8; Tlr4

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UniProt ID: (Mouse) Q9JHF9, (Mouse) Q9QUK6

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Entrez Gene ID: (Mouse) 17087, (Mouse) 21898

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功能
lipopolysaccharide receptor activity protein binding lipopolysaccharide binding receptor activity transmembrane signaling receptor activity phosphatidylinositol 3-kinase binding scaffold/adaptor protein
参与通路
pattern recognition receptor signaling pathway immune system process inflammatory response lipopolysaccharide-mediated signaling pathway positive regulation of lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide innate immune response activation of MAPK activity microglial cell activation activation of innate immune response toll-like receptor signaling pathway B cell proliferation involved in immune response nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway innate immune response-activating signal transduction leukotriene metabolic process response to oxidative stress signal transduction activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation response to bacterium positive regulation of platelet activation positive regulation of B cell proliferation interferon-gamma production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade toll-like receptor 4 signaling pathway macrophage activation positive regulation of NF-kappaB import into nucleus positive regulation of tumor necrosis factor biosynthetic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of DNA binding positive regulation of interleukin-12 biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of interferon-beta biosynthetic process positive regulation of interleukin-1 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol mast cell activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of lymphocyte proliferation regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process regulation of sensory perception of pain intestinal epithelial structure maintenance positive regulation of macrophage cytokine production negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway response to fatty acid cellular response to lipopolysaccharide cellular response to lipoteichoic acid
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