Invitrogen

TPH2 Polyclonal Antibody

Name: TPH2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-778

高级验证

12个已发表图片

18 篇参考文献

查看其他12个TPH2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

TPH2 Antibody in Immunoprecipitation (IP)

图: 1 / 14

TPH2 Antibody (PA1-778) in IP

Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (Product # PA1-778). Product # PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody ... View More

TPH2 Antibody in Immunohistochemistry (IHC)

TPH2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

TPH2 Antibody — Figure 1 Polymerase chain reaction and immunocytochemistry results. (A) Expression of the Cre (left) and TPH2 (right) marker genes. (B) Immunofluorescence in 5-HT-positive neurons in the wild-type group (magnification, x20). (C) 5-HT-positive neurons were lost in the TPH2-knockout group (magnification, x20). Scale bar=20 mum. Mlf, medial longitudinal fasciculus; 5-HT, 5-hydroxytryptamine; TPH2, tryptophan hydroxylase 2. {KO}

Immunogen image

Immunogen Image TPH2 Polyclonal Antibody

产品信息

PA1-778

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看9篇已发表文章 9篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:500

免疫沉淀 (IP)

1:1,000

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.1% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2207687

产品详细信息

PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples.

PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2.

The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.

靶标信息

Tryptophan hydroxylase (TPH) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. TPH is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。