Invitrogen

UCP3 Polyclonal Antibody

Name: UCP3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-055

高级验证

10个已发表图片

27 篇参考文献

查看其他6个UCP3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

图: 1 / 12

UCP3 Antibody (PA1-055) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of L6 (Lane 1) and C2C12 (Lane 2). The blot was probed with Anti-UCP3 Rabbit Polyclonal Antibody (Product # PA1-055, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 34 kDa band corresponding to UCP3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® ; 12% Bis-... View More

Product Image Gallery

UCP3 Antibody — Fig. 3. Long-chain acyl-CoA dehydrogenase (LCAD) and cytochrome c protein levels greater in animals fed a high-fat diet and given AICAR than either individual treatment in the white quadriceps. A : cytochrome c protein levels in skeletal muscle ( n = 7-10). B : LCAD protein levels in skeletal muscle ( n = 7-11). C : uncoupling protein 3 (UCP3) protein levels in skeletal muscle ( n = 6-10). D : representative Western blots. Letters are used to represent significance; same letter means no significant difference ( P < 0.05). AICAR and HF were not compared. *Main treatment effect ( P < 0.05). {TM}

Immunogen image

Immunogen Image UCP3 Polyclonal Antibody

产品信息

PA1-055

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

查看26篇已发表文章 26篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Dog, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UCP-3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2213925

产品详细信息

PA1-055 detects uncoupling protein-3 (UCP-3) mouse, rat, and canine tissues. This antibody will also detect recombinant human UCP-3, but does not detect UCP-3 from mouse liver or kidney.

PA1-055 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.

The PA1-055 immunogen is a synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UPC-3. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. PA1-055 immunizing peptide (Cat. # PEP-074) is available for use in neutralization and control experiments.

靶标信息

The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: mitochondrial uncoupling protein 3; Putative mitochondrial transporter UCP3; Solute carrier family 25 member 9; UCP 3; uncoupling protein; Uncoupling protein 3; Uncoupling protein 3, mitochondrial; Uncoupling protein-3

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基因别名: AI645527; SLC25A9; UCP-3; UCP3

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UniProt ID: (Dog) Q9N2I9, (Mouse) P56501, (Rat) P56499

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Entrez Gene ID: (Dog) 403575, (Mouse) 22229, (Rat) 25708

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功能

oxidative phosphorylation uncoupler activity hydrogen ion transmembrane transporter activity transporter secondary carrier transporter

参与通路