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Invitrogen
USP13 Polyclonal Antibody
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FIGURE: 1 / 2
USP13 Antibody (PA5-23318) in IHC (P)
Immunohistochemistry analysis of USP13 in formalin-fixed, paraffin-embedded human testis. Samples were incubated with USP13 polyclonal antibody (Product # PA5-23318) using a dilution of 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Product Details
PA5-23318
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Horse,
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 500-550 of human USP13.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is reactive to Chimpanzee, Rhesus Monkey.
Target Information
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin thioesterase 13; ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13
Gene Aliases: 2700071E21Rik; AI848077; IsoT-3; ISOT3; USP13
UniProt ID: (Bovine) E1BMF7, (Human) Q92995, (Mouse) Q5BKP2
Entrez Gene ID: (Bovine) 100141289, (Horse) 100065844, (Human) 8975, (Dog) 478640, (Mouse) 72607
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
zinc ion binding
ubiquitin protein ligase binding
ubiquitin binding
chaperone binding
proteasome binding
BAT3 complex binding
Lys48-specific deubiquitinase activity
protein binding
ubiquitin-like protein ligase binding
ubiquitin-specific protease activity involved in positive regulation of ERAD pathway
peptidase activity
cysteine-type peptidase activity
omega peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
cysteine protease
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
autophagy
cell proliferation
regulation of autophagy
protein K29-linked deubiquitination
protein K6-linked deubiquitination
protein stabilization
protein K63-linked deubiquitination
protein K48-linked deubiquitination
positive regulation of ERAD pathway
maintenance of unfolded protein involved in ERAD pathway
melanocyte differentiation
proteolysis
protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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