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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Ubiquitin Antibodies

          Invitrogen

          Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience™

          2个已发表图片
          16 篇参考文献
          查看其他49个Ubiquitin抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience™

          • 抗体检测数据 (3)
          • 已发表图片 (2)
          Ubiquitin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Ubiquitin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 5

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          Ubiquitin Antibody (14-6078-82) in WB

          Western blot was performed using Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience™ (Product # 14-6078-82) and ~80-260 kDa bands corresponding to poly ubiquitinated proteins were observed across the cell lines tested and increased upon treatment with MG132. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with 10 µM of MG132 for 90 minutes (Lane 2), HEK-293 (Lane 3) and HEK-293 treated with 10 µM of MG132 for 90 minutes (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX),... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Ubiquitin Antibody in Western Blot (WB)
          Ubiquitin Antibody in Western Blot (WB)
          Ubiquitin Antibody in Western Blot (WB)
          Ubiquitin Antibody in Western Blot (WB)
          Ubiquitin Antibody in Western Blot (WB)

          产品信息

          14-6078-82

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1-2 µg/mL
          查看9篇已发表文章 9篇已发表文章

          免疫组化 (IHC)

          Assay-Dependent
          -

          酶联免疫吸附实验 (ELISA)

          -
          查看2篇已发表文章 2篇已发表文章

          免疫沉淀 (IP)

          Assay-Dependent
          -

          亲和纯化 (AP)

          -
          查看5篇已发表文章 5篇已发表文章
          产品规格

          种属反应

          All, Human, Mouse, Rat

          已发表种属

          C. elegans, Human, Mouse, Plant, Yeast

          宿主/亚型

          Mouse / IgG1

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          eBioP4D1 (P4D1)

          偶联物

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • Request custom conjugation

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_837154

          产品详细信息

          Description: The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin.

          Applications Reported: This eBioP4D1 (P4D1) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

          Applications Tested: This eBioP4D1 (P4D1) antibody has been tested by immunblotting of treated cell lines. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; Ribosomal protein eS27-like; ribosomal protein S27; ribosomal protein S27-like; RPL40; RPS27A; RPS27L; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin/60S ribosomal fusion protein

          查看更多 收起

          基因别名: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80

          查看更多 收起

          UniProt ID: (Rat) P62982, (Human) P62979, (Human) Q71UM5, (Mouse) P62983, (Rat) P24051, (Mouse) Q6ZWY3, (Human) P62987, (Human) P0CG47, (Human) P0CG48, (Rat) P62986, (Mouse) P62984, (Rat) P0CG51, (Mouse) P0CG49, (Mouse) P0CG50, (Rat) Q63429

          查看更多 收起

          Entrez Gene ID: (Rat) 100912032, (Human) 6233, (Human) 51065, (Mouse) 78294, (Rat) 681429, (Mouse) 67941, (Human) 7311, (Human) 7314, (Human) 7316, (Rat) 64156, (Mouse) 22186, (Rat) 192255, (Mouse) 22187, (Mouse) 22190, (Rat) 50522

          查看更多 收起

          功能
          structural constituent of ribosome protein binding poly(A) RNA binding metal ion binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process protease binding ribosomal protein translational protein
          参与通路
          G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade nuclear-transcribed mRNA catabolic process, nonsense-mediated decay activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly viral transcription translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator ribosomal small subunit assembly DNA repair activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation biological_process positive regulation of defense response to virus by host cellular protein modification process xenophagy positive regulation of protein ubiquitination mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential regulation of proteasomal protein catabolic process regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination ubiquitin homeostasis male meiosis I female meiosis I male gonad development female gonad development hypothalamus gonadotrophin-releasing hormone neuron development adipose tissue development fat pad development seminiferous tubule development energy homeostasis ubiquitin-dependent protein catabolic process
          It has to be done as per old AB suggested Products section.
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